New Disease Reports
New Disease Reports (2016) 33, 17. [http://dx.doi.org/10.5197/j.2044-0588.2016.033.017]
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First report of Tobacco leaf curl Cuba virus infecting common bean in Cuba

R.M. Leyva 1, M.L. Quiñones 2*, K.I. Acosta 3, B. Piñol 2, C.D. Xavier 4 and F.M. Zerbini 4

*madeqp@censa.edu.cu

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Received: 05 Apr 2016; Published: 20 Apr 2016

Keywords: begomovirus, emerging pathogen, Phaseolus vulgaris

In Cuba begomoviruses have emerged as one of the major factors limiting production of solanaceous and fabaceous crops. The begomovirus Bean golden yellow mosaic virus affects the major production areas of common bean (Phaseolus vulgaris) in the country (Echemendía et al., 2001). Recently there has been an increase in the range of symptoms in beans, suggesting that other begomoviruses may be present.

During surveys of four fields of common bean in the province of Villa Clara (central Cuba) between November and December 2014, approximately 10% of plants exhibited begomovirus-like symptoms (Figs. 1, 2), including yellow mosaic, dwarfing, leaf blistering and abortion of flowers. A total of 23 leaf samples were collected and total DNA was extracted. Conventional PCR using universal primers PaL1v1978/PAR1c496 (Rojas et al., 1993) and rolling circle amplification combined with restriction fragment length polymorphism analyses showed begomoviruses to be associated with 80% of the symptom-bearing plants.

HindIII fragments of ~2.6 kb were ligated in the pBluescript KS(+) plasmid vector, previously cleaved with the same enzyme, and inserts of eight clones (two each from four samples) were sequenced. DNA-A sequences were obtained which shared 99.5% nucleotide sequence identity. The sequence of one presumed full-length clone (GenBank Accession No. KU562963), comprising 2610 nucleotides, showed the highest nucleotide identity (97%) with Tobacco leaf curl Cuba virus (TbLCCuV; AM050143) by BLAST analysis. Based on the begomovirus species demarcation criteria (Brown et al., 2015) the virus is an isolate of TbLCCuV.

TbLCCuV has previously been detected in tobacco in Cuba (Morán et al., 2006). Other begomoviruses are known to infect beans including Bean calico mosaic virus, Bean dwarf mosaic virus, Bean golden mosaic virus (Morales, 2011), Soybean blistering mosaic virus and Tomato yellow spot virus (Rodríguez‐Pardina et al., 2011). To our knowledge, this is the first report of TbLCCuV infecting common bean.

Figure1+
Figure 1: Common bean plants showing severe yellow mosaic on leaves.
Figure 1: Common bean plants showing severe yellow mosaic on leaves.
Figure2+
Figure 2: Common bean plants showing severe yellow mosaic, dwarfing and blistering on leaves, and abortion of flowers.
Figure 2: Common bean plants showing severe yellow mosaic, dwarfing and blistering on leaves, and abortion of flowers.

Acknowledgements

This work was supported by the CAPES-MES grant 155/12.

References

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To cite this report: Leyva RM, Quiñones ML, Acosta KI, Piñol B, Xavier CD, Zerbini FM, 2016. First report of Tobacco leaf curl Cuba virus infecting common bean in Cuba. New Disease Reports 33, 17. [http://dx.doi.org/10.5197/j.2044-0588.2016.033.017]

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